A polymorphic DNA probe from chromosome 7 (7q22)

A new polymorphic probe on chromosome 22: NB17 (D22S181).

Source/Description: NB17 is a 0.7 kb HindlO-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and was subcloned into pUC9. Polymorphism: Bgin or TaqI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: Bgin (Al: 12.5 kb, A2: 4.0 kb) with a constant band at 3.0 kb; TaqI (Bl: 2.9 kb, B2: 2.2 kb). The ...

A new polymorphic probe on chromosome 22: NB84 (D22S183).

Source/Description: NB84 is a 1.0 kb Hindm-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and is subcloned into pUC9. Polymorphism: PstI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: 4.6 kb (Al) and 4.0 kb (A2). No constant bands were present. Chromosomal Localization: Regional localization...

Isolation of polymorphic DNA fragments from human chromosome 4.

We have identified and characterized 40 DNA probes detecting restriction fragment length polymorphism (RFLP) on human chromosome 4. Single copy human clones were isolated from a bacteriophage library enriched for chromosome 4 sequences. Each clone was hybridized to somatic cell hybrid DNAs for verification of its species and chromosomal origin and for regional localization. Sequences specific f...

Isolation of polymorphic DNA segments from human chromosome 21.

A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction fragment length polymorphisms to provide genetic markers on chromosome 21. Ni...

YM40 (D2S75) is a polymorphic DNA sequence on chromosome 2.